Hi Ankit;

Please feel free to submit your DIA-Umpire questions and issues to our 
DIA-Umpire issue tracker: https://github.com/Nesvilab/DIA-Umpire

Regards

On Wednesday, April 3, 2019 at 7:05:59 AM UTC-4, Ankit Balhara wrote:
>
> Hi All,
>
> I am analyzing the SWATH-MS data using the DIA-Umpire. And I have 3 data 
> files of same sample which lead to generation of 9 .mgf output files from 
> DIA-Umpire, which were converted into 9 mzXML and then subjected to Comet 
> and X! Tandem search, and subsequently to peptide prophet and hence total 
> files generated during this process are 18 pep.xml files. 
> My ultimate aim to build the assay library. So, I am confused how to 
> combine the results of these 18 peptideprophet files.
>
> Should I combine the Comet search results of each data file separately and 
> X! Tandem results separately and then combine these outputs again using 
> iProphet? This should result in generation of 3 iprophet result files 
> corresponding to each data file, which can again combined into one file by 
> iProphet in final step?
>
> Or there is another way out of this problem?
>    
> Thanks
>
> Ankit
>

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