I'm running into the following error when trying to use xinteract to process Kojak 2.0 results. The command I'm running is: /usr/local/tpp/bin/xinteract -Ninteract.pep.xml -p0 -l1 -eT -D/path/to/database.fasta -PPM -OAPdp -drandom_ -ip ./kojak-output.pep.xml
INFO: Processing xl MixtureModel ... PeptideProphet (TPP v5.2.1-dev Flammagenitus, Build 201908090730-7945 (Linux-x86_64)) AKeller@ISB read in 0 1+, 34 2+, 11290 3+, 7625 4+, 2948 5+, 874 6+, and 13 7+ spectra. Initialising statistical models ... Found 13541 Decoys, and 9243 Non-Decoys Iterations: DEBUG HERE ... ERROR: nan detected in NonParametricDistribution ... command "/usr/local/tpp/bin/PeptideProphetParser 'interact.pep.xml' MINPROB=0 PPM ACCMASS NONPARAM DECOYPROBS DECOY=random_" exited with non-zero exit code: 256 QUIT - the job is incomplete This was previously working for me using TPP v5.2.0-b1 Flammagenitus Pre-release Beta, Build 201809101205-7809 (Linux-x86_64)) INFO: Processing xl MixtureModel ... PeptideProphet (TPP v5.2.0-b1 Flammagenitus Pre-release Beta, Build 201809101205-7809 (Linux-x86_64)) AKeller@ISB read in 0 1+, 34 2+, 11290 3+, 7625 4+, 2948 5+, 874 6+, and 13 7+ spectra. Initialising statistical models ... Found 13541 Decoys, and 9243 Non-Decoys Iterations: .........10.........20...... WARNING: Mixture model quality test failed for charge (1+). WARNING: Mixture model quality test failed for charge (2+). WARNING: Mixture model quality test failed for charge (7+). model complete after 27 iterations INFO: Processing loop MixtureModel ... etc. If anyone has any ideas why I am running into this error with the new build I'd be very grateful! Thanks! Alex -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to [email protected]. To view this discussion on the web visit https://groups.google.com/d/msgid/spctools-discuss/5436d7d4-3810-4af0-b32d-c432ebd91c5e%40googlegroups.com.
