Dear David
Thank you for your answer!, My question was regards this kind of data  from 
MALDI, with no a huge number of spectra. I have diffent dataset but I think 
my specific question was answered with you explanation.

Thank you again I´m sure I will have more doubts in the future.
Regards

Carlos


El lunes, 20 de julio de 2020, 21:53:20 (UTC-3), David Shteynberg escribió:
>
> Hi Carlos,
>
> The TPP is statistical software that takes advantage of statistical trends 
> in data and requires many data points to work well.  Do you have a dataset 
> of reasonable size with maybe dozens or hundreds of spectra?  Also can you 
> post the file somewhere I can download it?  Sending files as email 
> attachments may not work for larger files.
>
> Thanks,
> -David
>
> On Mon, Jul 20, 2020 at 5:12 PM Carlos Humberto Paván <[email protected] 
> <javascript:>> wrote:
>
>> Dear David
>> Thank you your answer and your offer. Of course I can send you the 
>> dataset. I have attached it. You will note this is a small number of 
>> spectrums.
>> Looking forward your comments
>>
>> PS I have attached the interact file too with the 0 prob result.
>>
>> El lunes, 20 de julio de 2020, 13:29:42 (UTC-3), David Shteynberg 
>> escribió:
>>>
>>> Dear Carlos, 
>>>
>>> There is probably some bug exposed by the modeling of this type of data 
>>> with the specific set of options you enabled.   If you are able to share a 
>>> part of your dataset that can replicate the issue, I am able to 
>>> troubleshoot this  further. 
>>>
>>> Thanks,
>>> David
>>>
>>> On Sat, Jul 18, 2020, 12:10 PM Carlos Humberto Paván <[email protected]> 
>>> wrote:
>>>
>>>> Dear all
>>>>
>>>> I have recently installed TTP 5.2 and I´m still learn about it. I´m 
>>>> stuck at the peptide validation process: I get the follow error next to 
>>>> run 
>>>> Peptide Prophet:
>>>> "Initialising statistical models ...
>>>>
>>>> command "C:/TPP/bin/PeptideProphetParser 
>>>> "/tmp/a04412/params/interact.pep.xml" MINPROB=0.0 CLEVEL=-1 PPM MALDI" 
>>>> failed: Unknown error
>>>>
>>>> command "C:/TPP/bin/PeptideProphetParser 
>>>> "/tmp/a04412/params/interact.pep.xml" MINPROB=0.0 CLEVEL=-1 PPM MALDI" 
>>>> exited with non-zero exit code: 255
>>>> QUIT - the job is incomplete"
>>>>
>>>> The data is from a 4800 MALDI, a single spot from gel.
>>>>
>>>> Regards and Thanks
>>>>
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