Dear Phillip, Can you please explain why I am getting this message during Xintreact processing. although its hase in the last processed all data.
running: "C:/TPP/bin/InteractParser "/tmp/a04564/params/Arvind/interact.pep.xml" "SF2.mzML.gz.pep.xml" -L"7"" file 1: SF2.mzML.gz.pep.xml Unknown .gz file. Only .mzML.gz and .mzXML.gz allowed. No file loaded.WARNING: cannot open data file c:\TPP\data\Arvind\TMT_saliva_6plex\SF2.gz in msms_run_summary tag... unrecognized extension .gz, trying .mzML ...WARNING: CANNOT correct data file c:\TPP\data\Arvind\TMT_saliva_6plex\SF2.mzML in msms_run_summary tag... Unknown .gz file. Only .mzML.gz and .mzXML.gz allowed. No file loaded.WARNING: cannot open data file c:\TPP\data\Arvind\TMT_saliva_6plex\SF2.gz in msms_run_summary tag... unrecognized extension .gz, trying .mzML ...WARNING: CANNOT correct data file c:\TPP\data\Arvind\TMT_saliva_6plex\SF2.mzML in msms_run_summary tag... processed altogether 28219 results On Mon, Dec 21, 2020 at 11:55 AM Phillip Wilmarth <pwilm...@gmail.com> wrote: > Hi Sud, > Here is a Comet parameters file with the TMT tags set as static > modifications (see bottom of the file): > > https://github.com/pwilmart/Metaplastic-BC_PXD014414/blob/master/comet.params > The tags are +229.1629 Da on the peptide N-term and on lysine residues. I > have an open source pipeline that uses Comet and supports TMT labeling: > https://github.com/pwilmart/PAW_pipeline > There are also many other resources at my GitHub site for TMT analyses. > Cheers, > Phil > > On Sunday, December 20, 2020 at 9:30:16 PM UTC-8 kumarsu...@gmail.com > wrote: > >> Can anyone please hare with me The *"comet search parameters for TMT >> 6plex labeled tryptic peptides*??? >> It will be a great help. >> Regards, >> Sud >> > -- > You received this message because you are subscribed to a topic in the > Google Groups "spctools-discuss" group. > To unsubscribe from this topic, visit > https://groups.google.com/d/topic/spctools-discuss/JAH2WjPJmU0/unsubscribe > . > To unsubscribe from this group and all its topics, send an email to > spctools-discuss+unsubscr...@googlegroups.com. > To view this discussion on the web visit > https://groups.google.com/d/msgid/spctools-discuss/af6d08ea-6eb3-475a-ae09-589b37ecd6fcn%40googlegroups.com > <https://groups.google.com/d/msgid/spctools-discuss/af6d08ea-6eb3-475a-ae09-589b37ecd6fcn%40googlegroups.com?utm_medium=email&utm_source=footer> > . > -- ------------------------------------------------------------------- The real voyage of discovery consists not in seeking new lands but seeing with new eyes. — Marcel Proust Dr. Sudarshan Kumar (B.V.Sc.& A.H., M.V.Sc., PhD.) Scientist Animal Biotechnology Center (Proteomics and Cell Biology Lab.) National Dairy Research Institute Karnal, 132001 Haryana, India Contact No 09254912456 URL www.ndri.res.in Orcid Id: https://orcid.org/0000-0002-9816-4307 -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to spctools-discuss+unsubscr...@googlegroups.com. To view this discussion on the web visit https://groups.google.com/d/msgid/spctools-discuss/CALZrgHSohdMkx-_pksqC-qP32TnTtEB44piGqWCrhK6%2BBu%3DE%2Bg%40mail.gmail.com.
