Paul, Thanks for the reply. I like the idea of treating a sample of clean BD w. the antimicrobial and then "inoculating it" with the cloudy stuff. I'll give it a try. My local auto parts store was all out of diesel antimicrobe stuff ... will get more in on Monday. When I asked if microbes were a common problem in diesel fuel, I was told that it's not common, but they do sell "quite a bit of the stuff" (antimicrobial). They were aware of filters getting clogged "by little buggers", but didn't know if sales were to treat a problem or the prevent one. It stands to reason that if dino diesel is on the menu for some bacteria/fungi, and biodiesel is more biodegradable than dino diesel, it may well be in the metabolic repertoire of a greater variety, or at least more common microbes. I think that if the problem is microbes, it is good news. I was worried that my WVO was the problem ... maybe some new additive screwing up the works. I have made more than thirty 20gal (76L) batches w/o any problem. Others have probably made 100's of batches w/o cultivating microbes. If it is, in fact, microbes in the brew, I suspect they're in my wash tank . The reactor and settling tanks seem too hostile an environment for a soil or water contaminant to survive. I'll scrub the wash tank, run a batch w. oil from the same settling tank and see what happens. Thanks again, Tom
----- Original Message ----- From: "Paul S Cantrell" <[EMAIL PROTECTED]> To: <Biofuel@sustainablelists.org> Sent: Saturday, March 18, 2006 9:39 PM Subject: Re: [Biofuel] Microbes in BD > Tom, > It's all those little mad cows swimming around ;-) > > Seriously, have you tried treating the 'innoculated' jar with the > anti-microbial to see if it clears up? Or, try a third jar that is > innoculated and treated with anti-microbial? > > Is there a microbiologist on the list? > > On 3/18/06, Thomas Kelly <[EMAIL PROTECTED]> wrote: >> >> Hello All, >> I suspect I have microbes in my recent batches of BD. >> After washing, I drained the BD and allowed it to settle. After a few >> days I >> noticed a whispy sediment on the bottom of the containers of fuel. I gave >> the batch another wash and cleaned my 5 gal. settling containers. The >> wash >> water was clear, but again the whispy sediment appeared after a few days. >> My next batch seemed to wash very well, but again, a whispy sediment >> can be observed after the BD is drained and allowed to settle. >> I brought a sample of the sediment to a local high school. We >> prepared >> a stained slide and observed a multitude of tiny uniformly-shaped spheres >> at >> 400X. >> I put 1 drop of the sediment in a glass jar w. 250ml of clear, >> uncontaminated BD and put 250ml of the same clear BD in an identical >> glass >> jar (control). Less than 24 hrs. later the "innoculated" jar is slightly >> cloudy w. a very fine sediment on the bottom and the control jar >> continues >> to be clear. >> - I started using WVO that includes some tallow. I noticed a post >> from JJN on 3/17/06 Re: Tallow: >> "I am treating all my bio with both an anti fungal and anti oxidant >> treatment since I use tallow alot." >> >> 1. Any thoughts/similar experience? >> 2. If it's microbes of some sort, should I treat the fuel w. a >> diesel anti-microbial and then filter it? >> Will a 10 micron filter remove these critters? >> 3. If microbes are present, do I have to be concerned w. >> >> metabolic byproducts screwing up the fuel? >> >> 4. If not microbes, what's the whispy stuff? >> >> Thanks, >> Tom > > > -- > Thanks, > PC > > He's the kind of a guy who lights up a room just by flicking a switch > > You can't have everything. Where would you put it? - Steven Wright > > _______________________________________________ > Biofuel mailing list > Biofuel@sustainablelists.org > http://sustainablelists.org/mailman/listinfo/biofuel_sustainablelists.org > > Biofuel at Journey to Forever: > http://journeytoforever.org/biofuel.html > > Search the combined Biofuel and Biofuels-biz list archives (50,000 > messages): > http://www.mail-archive.com/biofuel@sustainablelists.org/ > > > _______________________________________________ Biofuel mailing list Biofuel@sustainablelists.org http://sustainablelists.org/mailman/listinfo/biofuel_sustainablelists.org Biofuel at Journey to Forever: http://journeytoforever.org/biofuel.html Search the combined Biofuel and Biofuels-biz list archives (50,000 messages): http://www.mail-archive.com/biofuel@sustainablelists.org/