2006/9/4, CARVAJAL BARRIGA ENRIQUE JAVIER <[EMAIL PROTECTED]>:
I have done my Phd thesis making cellulase enzyme using Fungus Trichoderma Verde , during 1977 and 1983, the best especie selected by very famous researcher Mary Mandel , US Natic Army research laboratory who is very kind to send the same for several resarch institute to all the world
and latter renamed as Trichoderma Ressei
It is not practical one, eventhough can be used as pretreatmento for delignication before using enzymatic hydrolysis
During fermentation , the operating conditions for the cell growth for extraceluar enzyme growth need to me maintained and this is not the ideal one for the enzymatic hydrolysis. Moreover the sugar will be consumed for the cell growth .
The crude culture can be used with out the need for purification and concentration of the enzymes.
The reuse of enzymes are possible using new substrates , making possible the reduced consumption.
However the pretreatment need to be realized, as any impurities of the substarte can be inhibits enzymes.
We can send useful information to any one of our group.
Keith, has already longe before told me to write about ethanol from cellulose and some very useful information have been also available as post in this list
sd
Panniselvam
Thanks Juan:
I will be asking for enzymes and also I will try to use some fungus to
hydrolysate the substrate.
I have done my Phd thesis making cellulase enzyme using Fungus Trichoderma Verde , during 1977 and 1983, the best especie selected by very famous researcher Mary Mandel , US Natic Army research laboratory who is very kind to send the same for several resarch institute to all the world
I am not sure about the efficiency of using fungus, because I think they
are hydrolysating the woods for their own metabolism, and probably it
will diminish the amount of sugars to be converted into ethanol.
It is not practical one, eventhough can be used as pretreatmento for delignication before using enzymatic hydrolysis
During fermentation , the operating conditions for the cell growth for extraceluar enzyme growth need to me maintained and this is not the ideal one for the enzymatic hydrolysis. Moreover the sugar will be consumed for the cell growth .
The crude culture can be used with out the need for purification and concentration of the enzymes.
The reuse of enzymes are possible using new substrates , making possible the reduced consumption.
However the pretreatment need to be realized, as any impurities of the substarte can be inhibits enzymes.
We can send useful information to any one of our group.
Keith, has already longe before told me to write about ethanol from cellulose and some very useful information have been also available as post in this list
sd
Panniselvam
In this
aspect, probably the use of purified enzymes increases the yield of
ethanol and, also may reduce the time of hydrolysis.
I'd like to know your opinion on that respect.
Kind regards,
Javier
-----Mensaje original-----
De: [EMAIL PROTECTED]
[mailto: [EMAIL PROTECTED]] En nombre de Juan Boveda
Enviado el: Viernes, 25 de Agosto de 2006 15:19
Para: 'biofuel@sustainablelists.org'
Asunto: Re: [Biofuel] ENZYMES
Hola Javier.
Check with your local industrial washing operators of stone washed denim
jeans.
Many of them might use stones and celullase. They might tell you the
supplier of those cellulases usually they are many times cheaper than
chemical reagents suppliers.
There are some brands of hemicellulase and cellulase some are provided
by
Novo from Denmark or from others companies.
The cellulases are external enzymes from many sources, mainly from
fungal
origin and some you might find in your backyard, some good ones for
lignocellulose are Pleurotus sp. some used in oriental dishes and
others
found in the white decay of woods, they might be cultivated immersed in
agitated liquid media with wood pulp and some others nutrients similar
to a
diluted fertiliser plus some yeast extract with a clean air pumped
inside
the liquid.
Best Regards.
Juan
-----Mensaje original-----
De: CARVAJAL BARRIGA ENRIQUE JAVIER [SMTP:[EMAIL PROTECTED]]
Enviado el: viernes 25 de agosto de 2006 10:42
Para: biofuel@sustainablelists.org
Asunto: [Biofuel] ENZYMES
To anyone who can help indicating me where can I find the appropriate
enzymes to totally or partially hydrolysate lygnocellulosic material
such as spent grain from breweries to become fermentable sugars looking
to further bioethanol attainment. And also If anyone is involved in a
similar project to exchange experiences.
Many thanks,
Javier Carvajal
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