On Friday, July 12, 2013 8:39:24 AM UTC+1, [email protected] wrote:
>
>
>
> On Friday, July 12, 2013 1:48:06 AM UTC+1, Christoph Junghans wrote:
>>
>> 2013/7/11  <[email protected]>: 
>> > Dear Users, 
>> > 
>> > I am running IBI - My system  involves 90 beads, protein chain. Each 
>> > iteration is 5 ns and I am running 50 of them - at the begining. What I 
>> see 
>> > is that the first RDF has 100 times higher maximum than the target RDF. 
>> In 
>> > the second iteration it has 50 time higher maximum... in the third one 
>> it 
>> > has 5000 higher. 
>> Have a look at the trajectory with vmd, it might be that your protein 
>> forms a globule and never opens up again. 
>>
>
> Yes, this is true! It forms a globule and stack in this conformation. That 
> means the interactions are is too attractive... Would you suggest something?
> I was thinking about using fully repulsive between acidic residues and 
> basic residues? And maybe exclude 1-4 interactions or divide them by 2?
>
> Steven
>

I have in my topology:
 [ defaults ]
; nbfunccomb-rule    gen-pairs    fudgeLJ    fudgeQQ
1    1    yes    1.0    1.0
 
I would turn off the 1-4 interactions so set op nrexcl to 4 and apply fully 
repulsive to acidic-acidic basic-basic. How about vdwradii.dat? Shall 
increase it as it is taken from default settings i guess. Would you try sth 
else?

Steven


>  
>
>>
>> > Is that normal to have such fluctuations? Or maybe I have wrong values 
>> of 
>> > kbT in my settings which is 
>> > 
>> >  <!-- 300*0.00831451 gromacs units --> 
>> >     <kBT>2.49435</kBT> 
>> The order seems ok. And VOTCA will cross-check it with the value in 
>> your mdp file. 
>> > 
>> > Please, advise. 
>> > 
>> > Steven 
>> > 
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>> > 
>> > 
>>
>>
>>
>> -- 
>> Christoph Junghans 
>> Web: http://www.compphys.de 
>>
>

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