2013/7/12  <[email protected]>:
>
>
> On Friday, July 12, 2013 8:39:24 AM UTC+1, [email protected] wrote:
>>
>>
>>
>> On Friday, July 12, 2013 1:48:06 AM UTC+1, Christoph Junghans wrote:
>>>
>>> 2013/7/11  <[email protected]>:
>>> > Dear Users,
>>> >
>>> > I am running IBI - My system  involves 90 beads, protein chain. Each
>>> > iteration is 5 ns and I am running 50 of them - at the begining. What I
>>> > see
>>> > is that the first RDF has 100 times higher maximum than the target RDF.
>>> > In
>>> > the second iteration it has 50 time higher maximum... in the third one
>>> > it
>>> > has 5000 higher.
>>> Have a look at the trajectory with vmd, it might be that your protein
>>> forms a globule and never opens up again.
>>
>>
>> Yes, this is true! It forms a globule and stack in this conformation. That
>> means the interactions are is too attractive... Would you suggest something?
>> I was thinking about using fully repulsive between acidic residues and
>> basic residues? And maybe exclude 1-4 interactions or divide them by 2?
>>
>> Steven
>
>
> I have in my topology:
>  [ defaults ]
> ; nbfunccomb-rule    gen-pairs    fudgeLJ    fudgeQQ
> 1    1    yes    1.0    1.0
>
> I would turn off the 1-4 interactions so set op nrexcl to 4 and apply fully
> repulsive to acidic-acidic basic-basic. How about vdwradii.dat? Shall
> increase it as it is taken from default settings i guess. Would you try sth
> else?
This is not my field of expertise, so I will leave the question to
somebody else.....

>
> Steven
>
>>
>>
>>>
>>>
>>> > Is that normal to have such fluctuations? Or maybe I have wrong values
>>> > of
>>> > kbT in my settings which is
>>> >
>>> >  <!-- 300*0.00831451 gromacs units -->
>>> >     <kBT>2.49435</kBT>
>>> The order seems ok. And VOTCA will cross-check it with the value in
>>> your mdp file.
>>> >
>>> > Please, advise.
>>> >
>>> > Steven
>>> >
>>> > --
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>>> >
>>> >
>>>
>>>
>>>
>>> --
>>> Christoph Junghans
>>> Web: http://www.compphys.de
>
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>



--
Christoph Junghans
Web: http://www.compphys.de

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