There isnt much information here! Funny that 3 chains are poor - does that mean one and a half heterodimers? I presume you have checked spacegroup? Zanuda will test to see if any higher symmetry is present.. Eleanor
On 29 April 2013 06:02, sonali dhindwal <sonali11dhind...@yahoo.co.in>wrote: > Dear All, > > We are working on a crystal structure with 12 molecules in an asymmetric > unit. It is a heteromer and each chain is made up of two type of chains. > Therefore there are 24 chains in an asymmetric unit. > > It is giving solution in P1. Rfactor and Rfree has reached 20.25 and 25.6 > at a resolution of 1.8Angstrom > > All the chains are refining well and have good electron density except 3 > chains which have very poor electron density in most of the region. > > Whereas, the crystal structure of variants of the same protein we have > solved earlier refined well and has very good electron density in all the > chains. > > What could be the possible reason for these three chains which are not > refining properly. Any suggestions will be very useful. > > Thanks and Regards > > -- > Sonali Dhindwal > > “Live as if you were to die tomorrow. Learn as if you were to live > forever.” >
