[ccp4bb] cannot reproduce crystals

[dusky dew]

Dear all,
I am trying to reproduce some protein crystals. The protein I am getting
after cutting the his tag is very pure. I am using the reported protein
concentration. The cofactor and EDTA needs to be added externally. The
condition has calcium acetate, peg 4k and sodium acetate buffer.
Unfortunately I am getting oil separation and light ppt. I have no clue
what is wrong. Please help!