'oil separation and light ppt' is not necesserily an indicator that
something is wrong with your crystallization condition. Actually,
there are quite a few proteins that only crystallize in conditions
with oil/water-like phase separation. Some crystals even appear WITHIN
the oily drops (e. g. Xylanase).
good luck!
Clemens
Zitat von dusky dew <duskyde...@gmail.com>:
Dear all,
I am trying to reproduce some protein crystals. The protein I am getting
after cutting the his tag is very pure. I am using the reported protein
concentration. The cofactor and EDTA needs to be added externally. The
condition has calcium acetate, peg 4k and sodium acetate buffer.
Unfortunately I am getting oil separation and light ppt. I have no clue
what is wrong. Please help!
