DMSO is a good solvent for superglue and won't dissolve your loops. -- Kevin Jude, PhD Structural Biology Research Specialist, Garcia Lab Howard Hughes Medical Institute Stanford University School of Medicine Beckman B177, 279 Campus Drive, Stanford CA 94305 Phone: (650) 723-6431
On Mon, Nov 16, 2020 at 8:51 AM Diana Tomchick < diana.tomch...@utsouthwestern.edu> wrote: > We no longer use the reusable bases for the same reasons, but we do re-use > our bases. If you use superglue to glue the pins into the bases, you can > remove the pins from the bases by soaking them in acetone. This works about > 75% of the time, especially if you are careful not to use too much > superglue when you glue the pin into the base. > > It goes without saying that the use of acetone will dissolve the loop, but > then the main reason we remove the pin is due to imperfections in the loop, > so no big deal. > > Whenever we see the motions that you describe, we postulate that somehow > the pins were damaged and they are loose in the base. Occasionally this > motion is ascribed to ice between the base of the pin and the magnetic > mount, but this happens very rarely in our experience. > > Diana > > ************************************************** > Diana R. Tomchick > Professor > Departments of Biophysics and Biochemistry > UT Southwestern Medical Center > 5323 Harry Hines Blvd. > Rm. ND10.214A > Dallas, TX 75390-8816 > diana.tomch...@utsouthwestern.edu > (214) 645-6383 (phone) > (214) 645-6353 (fax) > ------------------------------ > *From:* CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of Tao-Hsin > Chang <taohsin.ch...@gmail.com> > *Sent:* Sunday, November 15, 2020 3:07 PM > *To:* CCP4BB@JISCMAIL.AC.UK <CCP4BB@JISCMAIL.AC.UK> > *Subject:* Re: [ccp4bb] Stabilizing Mitegen reusable bases/mounts > > > EXTERNAL MAIL > > Hi Patrick, > > I have met the same issue and that is very bad for any micro-focus beam > and small crystals. I have talked with Mitegen and they are developing a > new type of reusable base having an improvement for this issue. You may get > some update from them. But, I do not use the reusable bases anymore. > > Best wishes, > Tao-Hsin > > On Nov 15, 2020, at 3:45 PM, Patrick Loll <pjl...@gmail.com> wrote: > > Hi everyone, > > I’ve become very fond of the Mitegen reusable bases for mounting crystals, > since the reusable aspect savse me from having to discard the base every > time I break a microloop. However, once the crystals arrive at the > synchroteon, I observe motions of the loops (some gradual, some sporadic). > The the amplitudes of these motions are becoming significant as I take data > from smaller and smaller crystals. I don’t think I’m imagining this, since > the good folks at NSLS-2/AMX have warned me about this very issue. > > I’m writing to ask if anyone has any clever ideas about stabilizing these > assemblies. Obviously, I can epoxy the pins in place, but then I’ll > probably need to discard the entire assembly when I break a loop, and I’d > prefer not to waste more money than necessary. I’ve considered putting a > bead of wax at the point where the pin enters the base (although I haven’t > yet checked to see if that will survive immersion in liquid nitrogen). Does > anyone have any other (better) ideas? > > Much obliged in advance, > > Pat > __________________________ > > Patrick J. Loll, PhD > Professor of Biochemistry & Molecular Biology > Drexel University College of Medicine > Room 10-102 New College Building > 245 N. 15th St. > Philadelphia, PA 19102-1192 USA > > (215) 762-7706 > pj...@drexel.edu > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > CAUTION: This email originated from outside UTSW. Please be cautious of > links or attachments, and validate the sender's email address before > replying. > > > ------------------------------ > > UT Southwestern > > Medical Center > > The future of medicine, today. > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
