[ccp4bb] Announcement: Molecular Graphics Software CueMol version 2.2

2014-11-05 Thread Ryuichiro Ishitani
Dear All, I'm sorry for off-topic posting. We are pleased to announce the molecular graphics program CueMol version 2.2. http://www.cuemol.org/en/ CueMol is an open-source computer program for the macromolecular structure visualization, especially for the sophisticated publication-quality

[ccp4bb] Reminder - CCP4 Study Weekend 2015 - Early bird registration and Standard Student bursary deadline is 24th November 2014

2014-11-05 Thread Karen McIntyre
The CCP4 Study Weekend (7 - 9 January 2015) East Midlands Conference Centre, University of Nottingham Wednesday 7 - MX User Meeting Thursday 8 / Friday 9 - CCP4 Study Weekend Advances in Experimental Phasing We cordially invite you to participate in the 2015 CCP4 Study Weekend at the the East

[ccp4bb] Open postdoc positions in research and scientific software development (EMBL Hamburg, Lamzin)

2014-11-05 Thread Philipp Heuser
Dear colleagues, I would like to make you aware of two open positions in the Lamzin group at the EMBL in Hamburg. We are looking for two enthusiastic postdoctoral fellows in research and scientific software development: 1) The post holder will play a key role in the development of the ARP/wARP

[ccp4bb] Career Development Fellow Birkbeck University of London

2014-11-05 Thread Nicholas Keep
Birkbeck, University of London has a long tradition in structural biology going back to JD Bernal. As part of the institute for Structural Molecular Biology http://www.ismb.lon.ac.uk/ under Prof Gabriel Waksman FRS, it remains a leading centre in the UK for structural biology. The college

[ccp4bb] The phase problem

2014-11-05 Thread Giulliana Rangel
Dear all, I would like to known if someone could help me with some idea about the phase problem. I am a beginner in crystallography and the first time I tryed to solve the structure by Molrep, amore, mr. Bump and I didnt find anything for molecular replacement. Thus, currently I've tried to

Re: [ccp4bb] The phase problem

2014-11-05 Thread Nicholas Larsen
Screen for heavy atoms with native gel. Run protein with variety of heavy atoms. If you see a discreet shift in the native gel, then you will get derivative. If you don't see a shift, don't bother soaking. It's worked for me every time. Also, ammonium sulfate will mess up your heavy atoms, so

Re: [ccp4bb] The phase problem

2014-11-05 Thread Andreas Förster
Hi Giuliana, if your crystal diffracts to 3 Å or better and you have at least one Cys/Met per 25 residues, put it on the home source and collect until the sulfur signal rises above the noise. Then solve by SAD. http://www3.imperial.ac.uk/xraycrystallography/learning/sulfur_sad Andreas

Re: [ccp4bb] The phase problem

2014-11-05 Thread Alessandro Nascimento
Hi Giulliana, I would (re)check for issues during the assignment of the space group prior to MR. Many people in the list can help you with this if you provide additional information regarding your data. In case you have a structure that can not be solved by MR, I would give a chance for the