Re: [ccp4bb] pseudo-centering or twinning problem in P3

Maybe, but it may also indicate that the problem isn’t twinning, but simply pseudo symmetry. Zanuda is one way to double-check, and it takes approximately a day to re-process the data, run the MR and run Zanuda. Diana ** Diana R. Tomchick

Re: [ccp4bb] pseudo-centering or twinning problem in P3

That seems hard work to me! Data quality suffers when you discard multiplicity.. Eleanor On 8 August 2017 at 17:15, Sudipta Bhattacharyya < sudiptabhattacharyya.iit...@gmail.com> wrote: > Hi Giorgio, > > I also suspect the presence of twining and as Eleanor has suggested the L > test is the

Re: [ccp4bb] doubt regarding setting resolution cutoff

You have some horrible ice rings - some data processing software may be able to cut them out.. how are you processing it? Eleanor On 8 August 2017 at 15:43, Christian Roth wrote: > Your plots look strangely different to the old Scala output you posted > before, but

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Re: [ccp4bb] pseudo-centering or twinning problem in P3

Hi Giorgio, I also suspect the presence of twining and as Eleanor has suggested the L test is the best test to detect that (if also t-NCS is not present). You can reprocess your data in P1, do MR in P1 and then feed your P1 model and mtz file to zanuda to see what SG it suggests. That trick once

Re: [ccp4bb] doubt regarding setting resolution cutoff

Your plots look strangely different to the old Scala output you posted before, but never mind. Paul is right that a negative intensity is not desired and your crystal has some issues with ice. That one icering around 2.26 must be massive taken into account how haywire your curve goes there.

Re: [ccp4bb] doubt regarding setting resolution cutoff

On 08/08/2017 15:07, Satvik Kumar wrote: Dear Prof. Powell and Prof. Dodson, Thanks for your reply and advise. As per your suggestion, I have re-scaled the intensities using Aimless at 1.861 A. I observe that the I/sigI has dropped to -0.8 That's not good. > and the behaviour of CC_1/2

Re: [ccp4bb] pseudo-centering or twinning problem in P3

First of all - check whether your crystal data shows twinning - thee is an L-test plot which usually gives a clear indication and if you are using GUI2 the report suggests if this is so. If you have twinning the most likely SG is P31 (or P32 ) - you cant tell the difference till the structure is

[ccp4bb] pseudo-centering or twinning problem in P3

Hello everybody, I think I have a pseudo-centering problem. I have a 1.6 ang. dataset of a mutant protein that is an homodimer in solution. Data processing gives the following SG: Space group: P 31 2 1 Average unit cell: 111.36 111.36 28.56 90.00 90.00 120.00 Average mosaicity: