[ccp4bb] Postdoctoral positions in cryo-EM of signalling complexes at UCSF

The Jura and Verba labs at the University of California San Francisco (UCSF) currently have two open postdoctoral positions to work on application of cryo-EM to the studies of signaling complexes. One project focuses on structural investigation of Receptor Tyrosine Kinase (RTK) complexes

Re: [ccp4bb] postdoctoral position in cryo-EM at LMB in Jan Löwe's group

Thank you Sir I had checked with Gaythri and applying them . Thanks and Regards Samar On Tue, 16 Feb, 2021, 10:32 PM Jan Löwe, wrote: > Dear all, > > My group has a grant-funded job opening for a postdoctoral structural > biologist to work on problems in archaeal and bacterial cell biology. The

[ccp4bb] Sr. Scientist position available at Merck South San Francisco- CryoEM and X-ray crystallography [Confidential]

Confidential Details to apply here: https://msd.wd5.myworkdayjobs.com/SearchJobs/job/USA---California---South-San-Francisco---Grand-Ave/Sr-Scientist--Structural-Biologist--Cryo-EM-and-X-ray-crystallography-_R100253 Job Description Our Research Scientists are our Inventors. We identify and

[ccp4bb] Amended link to application - Ph.D. position available at the University of Oxford-Centre for Medicines Discovery in partnership with AstraZeneca-Cambridge

Dear all, Apologies for re-posting. I would like to amend here the link to the application for the following Ph.D. position: We are recruiting a PhD student to investigate regulatory aspects of protein mega-complexes involved in the Integrated Stress Response (ISR), a signalling pathway

[ccp4bb] Post-Doctoral Position at the University of Illinois to Study Ribosome Structure

*Post-Doctoral Position in Structural Biology at the University of Illinois at Chicago (UIC) to Study the Structure and Function of the Ribosome* The Polikanov Laboratory at the University of Illinois at Chicago (IL, USA) is looking for a talented, skillful, and highly motivated researcher to

Re: [ccp4bb] strange crystals

Dear Dale, A warm thank you for pointing out what I had overseen. And a big thanks to all the others who commented so far, cheers, Gerlind On 16/02/2021 21:19, Dale Tronrud wrote: On 2/16/2021 11:47 AM, Gerlind Sulzenbacher wrote: Dear all, I wonder whether any of you have ever

Re: [ccp4bb] strange crystals

On 2/16/2021 11:47 AM, Gerlind Sulzenbacher wrote: Dear all, I wonder whether any of you have ever encountered such strange crystals. In the images you can download via the link below, crystal1 looks hollow, crystal2 looks like a cubic crystal embedded within a hexagonal arragement. I

Re: [ccp4bb] Challenging Molecular Replacement

Yes, I agree with Eleanor. But if there’s translational NCS, either the off-origin Patterson peak is below 20% of the origin peak so that Phaser is missing it, or perhaps you’re turning off the tNCS correction? If Phaser detects tNCS it will place two copies at once. We’ve had cases where,

[ccp4bb] strange crystals

Dear all, I wonder whether any of you have ever encountered such strange crystals. In the images you can download via the link below, crystal1 looks hollow, crystal2 looks like a cubic crystal embedded within a hexagonal arragement.

Re: [ccp4bb] Challenging Molecular Replacement

There are a large number of PAK clashes, I would regard this "solution" with caution. Have you inspected the solution to see where these clashes are occurring? If they are from long flexible loops or the termini, perhaps if you would trim the model to remove those clashes and re-run the MR

Re: [ccp4bb] Challenging Molecular Replacement

Hi Muhammad, It sounds like you are on the right track, but maybe you are in the wrong space group. You could check out Zanuda (part of CCP4). It will refine your solution in other potential space groups and compare the refinement statistics. Best of luck! Cheers, Jessica On Tue, Feb 16, 2021

Re: [ccp4bb] Challenging Molecular Replacement

Hi, Looks like, you have the solution. Have you tried the Phenix AutoBuild module? sometimes it works well. Best luck On Tue, Feb 16, 2021 at 1:21 PM Muhammad Bashir Khan wrote: > Dear All; > > I have data set of about 3.0A. It's a multidomain protein. I tried several > options with MR but

Re: [ccp4bb] Challenging Molecular Replacement

Well - your LLG etc looks good but those two solutions have symmetry equivalent rotation angles so must be related by a translation vector. SOLU 6DIM ENSE ense_1 EULER 269.0 80.4 177.3 FRAC 0.24 -0.22 -0.07 BFAC -1.51 #TFZ==6.5 SOLU 6DIM ENSE ense_1 EULER 89.0 80.4 177.3 FRAC

[ccp4bb] Challenging Molecular Replacement

Dear All; I have data set of about 3.0A. It's a multidomain protein. I tried several options with MR but it's was not working. I trimmed one of the search models, it does not give any solution using CCP4 molrep. I used Phenix phaser it gives a solution after several hours with the following

[ccp4bb] Open position in Salminen lab, Structural Bioinformatics Laboratory, Abo Akademi University, Turku, Finland

Dear all, My group has an opening for a two-year project researcher position within molecular mechanism of inflammation. The project aims for 3D structure determination of protein-protein and protein-ligand complexes enabling rational development of anti-inflammatory and anti-cancer agents.

[ccp4bb] postdoctoral position in cryo-EM at LMB in Jan Löwe's group

Dear all, My group has a grant-funded job opening for a postdoctoral structural biologist to work on problems in archaeal and bacterial cell biology. The aim is to understand cell division and vesicle formation at the molecular or even atomic scales. The work will involve state-of-the-art