Re: [ccp4bb] ccp4 development resources

Dear Lothar [I’ve copied this to the CCP4 team in case they have suggestions] I don’t think there is any specific guidance for writing a “CCP4 program” - the programs distributed in the suite have been written in a diversity of styles and languages over many years - but I can offer a few

[ccp4bb] A challenging Molecular replacement

I am a structural biologist who is teaching X-ray crystallography. Recently I noticed that BrlR structure (5XQL) was solved using molecular replacement with a search model of very low similarity. I am very interested in this structure because I think this a very good example to show students

Re: [ccp4bb] A challenging Molecular replacement

Richard, I can’t help you with 5XQL. However, I can point out a recent structure from my group that might be useful for teaching. The structure was solved by MR with a search model that had 33% sequence identity and represented only 46% of the target structure. The Methods section of the

Re: [ccp4bb] A challenging Molecular replacement

On Tuesday, 18 July 2017 00:01:59 CDaddy wrote: > I am a structural biologist who is teaching X-ray crystallography. Recently I > noticed that BrlR structure (5XQL) was solved using molecular replacement > with a search model of very low similarity. I am very interested in this > structure

[ccp4bb] suggestions are welcome

Hi all , It has been reported the Negative stain EM of a protein A-B complex, but according to my gel filtration results (I purified A and B respectively for incubation) , I found that A could not bind to B, of course I tried different buffer condition with various pH value, even the binding

Re: [ccp4bb] suggestions are welcome

Hi, This might indicate that the binding affinity of your complex is not high enough for it to resist the gel filtration dilution. Maybe running a native PAGE gel would allow you to visualise a complex. Best A > On 18 Jul 2017, at 10:25, 高艺娜 wrote: > > Hi all , > > It has

Re: [ccp4bb] A challenging Molecular replacement

Dear Dr.Richard, Your paper gave a good example for us. Is it possible that you send me all the related files? It will be very helpful for students to learn the detail of solving difficult protein structures. Thanks in advance! best, Zhonghao Chen China agricultural university.