[ccp4bb] 回复：[ccp4bb] suggestions are welcome

please characterize your a and b to see they really what they are 发自网易邮箱大师 在2017年07月18日 10:25，高艺娜 写道： Hi all , It has been reported the Negative stain EM of a protein A-B complex, but according to my gel filtration results (I purified A and B respectively for incubation) , I found that A

Re: [ccp4bb] Fine Phi Slicing

An important aspect of fine phi slicing that has not been mentioned yet (and took me a long time to figure out) is the impact of read-out time. Traditionally, read-out time is simply a delay that makes the overall data collection take longer, but with so-called "shutterless" data collection

[ccp4bb] Staraniso - determination of anisotropic resolution limits and statistics

Dear Gerard and Ian, thank you for your work in putting together the Staraniso sever and your effort to tackle the anisotropic diffraction problem, a non-trivial behavior that creates many problems in structure solving, and that is very prevalent in the world of membrane proteins... I have

[ccp4bb] XQuartz problems

Hello Mac Gurus, I have run today into a problem with XQuartz on my iMac. No problems till now. Now, any application that uses X11 does not start. It invokes XQuartz but that is it. What is more, when running, XQuartz can not be stopped; after an attempt to quit the application XQuartz

Re: [ccp4bb] Fine Phi Slicing

James, On On 20 Jul 2017, at 19:06, James Holton > wrote: In my experience you need at least an average of 1 photon/pixel/image before even the best data processing algorithms start to fall over. I do not agree… both XDS and

[ccp4bb] ample

I’m intrigued by the prospect of using AMPLE to test multiple distant homologs in a MR problem. I’ve used HHPRED to identify about 20 high-probability homologs of known structure, each of which has about 20-25% identity with the unknown protein. However, it’s not clear to me from the

[ccp4bb] high Rfree

Hi everyone I have got a anti-parallel coiled-coil structure in a short fragment recently, then I want to solve a longer fragment structure with phenix-MR using this short fragment structure as a model.The result is not good because of the Rwork and Rfree is high.So I think the longer

Re: [ccp4bb] Fine Phi Slicing

Based on this, a vision for the future: A warehouse filled with sealed-tube, top-hat-profiled sources, super-accurate goniostats, and Dectris detectors, a robot running back and forth from a central dewar to place the crystals, all images 1-bit, intensities measured as probabilities; a day