Re: [Histonet] Tissue falling off slides

2024-01-31 Thread Rachel Gonzalez via Histonet 
Hi
I used to have that problem.   A senior histologist from BioCare recommended to 
air dry the slides overnight and then bake them. I have not had the problem 
since.
Rachel

From: Ken M via Histonet 
Sent: Wednesday, January 31, 2024 6:07 PM
To: richard cartun ; histonet@lists.utsouthwestern.edu; 
Foshey, Annette 
Subject: Re: [Histonet] Tissue falling off slides

1) Try tap water
2) Try hydrophilic IHC slides, (I will send you samples for free)

Ken
kdea...@hotmail.com<mailto:kdea...@hotmail.com>


-Original Message-
From: richard cartun via Histonet 
mailto:histonet@lists.utsouthwestern.edu>>
Sent: Wednesday, January 31, 2024 10:29 AM
To: 
histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>; 
Foshey, Annette mailto:afos...@mcw.edu>>
Subject: Re: [Histonet] Tissue falling off slides

Hi Annette!
Do you put anything else in your water bath besides deionized water?
Richard CartunMorphologic Proteomics, LLC(860) 490-7633

On Tuesday, January 30, 2024 at 03:14:50 PM EST, Foshey, Annette via Histonet 
mailto:histonet@lists.utsouthwestern.edu>> 
wrote:


I work with whole mount prostate blocks that I cut and stain for IHC manually. 
I use superfrost plus slides that I purchase from the Brain Research 
Laboratories. I airdry the slides after sectioning and then they are placed in 
a rack and dried in a 60 degree oven overnight. On some of the slides the 
tissue still lifts off the slides usually it is where there is tumor. Any 
suggestions would be greatly appreciated.

Kindest Regards,
Annette Foshey, HT
Histology Technologist, Precision Medicine Laboratory (PML)
Phone: (414) 955-7537 | E-mail: 
afos...@mcw.edu<mailto:afos...@mcw.edu<mailto:afos...@mcw.edu%3cmailto:afos...@mcw.edu>>
Medical College of Wisconsin | 8701 W Watertown Plank Road, Milwaukee, WI 53226

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet<http://lists.utsouthwestern.edu/mailman/listinfo/histonet>

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet<http://lists.utsouthwestern.edu/mailman/listinfo/histonet>

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet<http://lists.utsouthwestern.edu/mailman/listinfo/histonet>

Disclaimer

The information contained in this communication from the sender is 
confidential. It is intended solely for use by the recipient and others 
authorized to receive it. If you are not the recipient, you are hereby notified 
that any disclosure, copying, distribution or taking action in relation of the 
contents of this information is strictly prohibited and may be unlawful.

This email has been scanned for viruses and malware, and may have been 
automatically archived by Mimecast Ltd, an innovator in Software as a Service 
(SaaS) for business. Providing a safer and more useful place for your human 
generated data. Specializing in; Security, archiving and compliance. To find 
out more visit the Mimecast website.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Tissue falling off slides

2024-01-31 Thread Ken M via Histonet 
1) Try tap water
2) Try hydrophilic IHC slides, (I will send you samples for free)

Ken
kdea...@hotmail.com


-Original Message-
From: richard cartun via Histonet 
Sent: Wednesday, January 31, 2024 10:29 AM
To: histonet@lists.utsouthwestern.edu; Foshey, Annette 
Subject: Re: [Histonet] Tissue falling off slides

 Hi Annette!
Do you put anything else in your water bath besides deionized water?
Richard CartunMorphologic Proteomics, LLC(860) 490-7633

On Tuesday, January 30, 2024 at 03:14:50 PM EST, Foshey, Annette via 
Histonet  wrote:


I work with whole mount prostate blocks that I cut and stain for IHC manually. 
I use superfrost plus slides that I purchase from the Brain Research 
Laboratories. I airdry the slides after sectioning and then they are placed in 
a rack and dried in a 60 degree oven overnight.  On some of the slides the 
tissue still lifts off the slides usually it is where there is tumor.  Any 
suggestions would be greatly appreciated.

Kindest Regards,
Annette Foshey, HT
Histology Technologist, Precision Medicine Laboratory (PML)
Phone: (414) 955-7537 | E-mail: afos...@mcw.edu<mailto:afos...@mcw.edu>
Medical College of Wisconsin | 8701 W Watertown Plank Road, Milwaukee, WI 53226

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Tissue falling off slides

2024-01-31 Thread richard cartun via Histonet 
 Hi Annette!
Do you put anything else in your water bath besides deionized water?
Richard CartunMorphologic Proteomics, LLC(860) 490-7633

On Tuesday, January 30, 2024 at 03:14:50 PM EST, Foshey, Annette via 
Histonet  wrote:  
 
 
I work with whole mount prostate blocks that I cut and stain for IHC manually. 
I use superfrost plus slides that I purchase from the Brain Research 
Laboratories. I airdry the slides after sectioning and then they are placed in 
a rack and dried in a 60 degree oven overnight.  On some of the slides the 
tissue still lifts off the slides usually it is where there is tumor.  Any 
suggestions would be greatly appreciated.

Kindest Regards,
Annette Foshey, HT
Histology Technologist, Precision Medicine Laboratory (PML)
Phone: (414) 955-7537 | E-mail: afos...@mcw.edu
Medical College of Wisconsin | 8701 W Watertown Plank Road, Milwaukee, WI 53226

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
  
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Tissue falling off slides

2009-11-10 Thread Weems, Joyce 
Put some alcohol in the water bath - it will reduce the surface tension.
Maybe that will help. j 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of John
Shelley
Sent: Tuesday, November 10, 2009 09:25
To: histonet@lists.utsouthwestern.edu
Cc: Betsy Woessner; Kathryn Gizzi
Subject: [Histonet] Tissue falling off slides

Good morning histonetters!!

I received a project from an outside source to process and embed mouse
hearts for this group. From what I could gather the specimens had
varying times in 4% paraformaldehyde from 24-48 hours all the way up to
4 months. Once processed and embedded the specimens where returned to
the group to cut. They are using superfrost plus slides from Fisherbrand
and from what I could see the sections were coming off fine. The only
thing I did notice is that when they put the slide in the water bath the
tissue seemed to be repelled which from experience means that too much
coating on slide and a hydrophobic environment is create. I asked them
to look for slides from a different lot and cut additional sections and
from those sections I asked them to air-dry only and others I asked for
them to place in oven for 30-45 mins. at 56 to 60 degrees or until they
did not see any more water droplets. They said the sections that they
placed in the oven stayed on a little longer in the deparaffinizing
steps but still fell off.  I know that it could be processing also but
the specimens looked great when I embedded them. Any suggestions will be
great, sometimes when your right on top of it you lose sight of other
possibilities.

John


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Confidentiality Notice:
This email, including any attachments is the 
property of Catholic Health East and is intended 
for the sole use of the intended recipient(s).  
It may contain information that is privileged and 
confidential.  Any unauthorized review, use,
disclosure, or distribution is prohibited. If you are 
not the intended recipient, please reply to the 
sender that you have received the message in 
error, then delete this message.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet