HI Henrik, I could able to resolve my problem wrt CDF files but now i am struck with following error. > cs <- AffymetrixCelSet$byName("tissues", cdf=cdf) Error in list(`AffymetrixCelSet$byName("tissues", cdf = cdf)` = <environment>, :
[2010-08-05 13:38:14] Exception: Cannot create AffymetrixCelSet. No such directory: tissues/HuGene-1_0-st-v1 at throw(Exception(...)) at throw.default("Cannot create ", class(static)[1], ". No such directory: ", path) at throw("Cannot create ", class(static)[1], ". No such directory: ", path) at method(static, ...) at AffymetrixCelSet$byName("tissues", cdf = cdf) > I would like to know what directory i should create for getting rid of this error Thanks Vish On Thu, Aug 5, 2010 at 1:10 PM, ashwin Vishnuvardhana < ashwin4bioinformat...@gmail.com> wrote: > HI but still I have a problem, These are steps i am doing > > 1. My current directory is on desktop where exactly my raw data is > 2. then I am trying to use the command in aroma.project > 3. I did changed the directory as you mentioned > > And the error remains same. I have one question do we convert cdf (text) > file to binary ? I downloaded it from aroma project website. > > Thanks > Vish > > > On Thu, Aug 5, 2010 at 12:38 PM, Henrik Bengtsson > <h...@stat.berkeley.edu>wrote: > >> Hi, >> >> you write: >> >> annotationData/Chip type/HuGene-1_0-st-v1/HuGene-1_0-st-v1,r3.CDF >> >> but that is *not* was is documented or written in the vignettes. You >> should have: >> >> annotationData/chipTypes/HuGene-1_0-st-v1/HuGene-1_0-st-v1,r3.CDF >> >> It is really important that you use the correct directory names >> exactly like that. >> >> /Henrik >> >> On Thu, Aug 5, 2010 at 7:34 PM, ashwin Vishnuvardhana >> <ashwin4bioinformat...@gmail.com> wrote: >> > HI Pierre, >> > I did saved in the similar way (aroma.affymetrix/annotationData/Chip >> > type/HuGene-1_0-st-v1/HuGene-1_0-st-v1,r3.CDF) >> > but still i am coming up with same error. >> > >> > Thanks >> > Vish >> > >> > On Tue, Aug 3, 2010 at 5:32 PM, Pierre Neuvial < >> pie...@stat.berkeley.edu> >> > wrote: >> >> >> >> Hi, >> >> >> >> Most probably you haven't put the required CDF file in the >> >> corresponding annotationData/chipTypes/ directory. >> >> >> >> Have you read the vignette http://aroma-project.org/node/38 ? This is >> >> well explained there. >> >> >> >> Also, similar versions of this question have been answered on this >> >> list, see for example >> >> >> >> >> http://groups.google.com/group/aroma-affymetrix/browse_thread/thread/d992f2ca51e71774/8ba42bb7bdbf1b48 >> >> >> >> Best >> >> >> >> Pierre. >> >> >> >> >> >> Be sure to put the CDF file in your annotationData/chipTypes/XX/ where >> >> XX is the platform you are using (e.g. HuGene-1_0-st-v1 for Human >> >> Gene 1.0 ST, HT_HG-U133_Plus_PM for the human PM plate arrays, etc.) >> >> >> >> On Tue, Aug 3, 2010 at 2:27 PM, vish <ashwin4bioinformat...@gmail.com> >> >> wrote: >> >> > >> >> > I am really new to this aroma.affymetrix. I am trying to analyze >> >> > HuGene-1_0-st-v1 array and interested in Quality control check/ >> >> > Assessment. >> >> > when I am trying to run this command I am ending up with this error . >> >> > Any help is greatly appreciated. >> >> > >> >> > cdf <- AffymetrixCdfFile$byChipType(chipType, tags="r3") >> >> > Error in list(`AffymetrixCdfFile$byChipType(chipType, tags = "r3")` = >> >> > <environment>, : >> >> > >> >> > [2010-08-03 16:23:44] Exception: Could not locate a file for this >> chip >> >> > type: HuGene-1_0-st-v1,r3 >> >> > at throw(Exception(...)) >> >> > at throw.default("Could not locate a file for this chip type: ", >> >> > paste(c(chipType, tags), collapse = ",")) >> >> > at throw("Could not locate a file for this chip type: ", >> >> > paste(c(chipType, tags), collapse = ",")) >> >> > at method(static, ...) >> >> > at AffymetrixCdfFile$byChipType(chipType, tags = "r3") >> >> > >> >> > Ashwin >> >> > >> >> > -- >> >> > When reporting problems on aroma.affymetrix, make sure 1) to run the >> >> > latest version of the package, 2) to report the output of >> sessionInfo() and >> >> > traceback(), and 3) to post a complete code example. >> >> > >> >> > >> >> > You received this message because you are subscribed to the Google >> >> > Groups "aroma.affymetrix" group with website >> http://www.aroma-project.org/. >> >> > To post to this group, send email to >> aroma-affymetrix@googlegroups.com >> >> > To unsubscribe and other options, go to >> >> > http://www.aroma-project.org/forum/ >> >> > >> >> >> >> -- >> >> When reporting problems on aroma.affymetrix, make sure 1) to run the >> >> latest version of the package, 2) to report the output of sessionInfo() >> and >> >> traceback(), and 3) to post a complete code example. >> >> >> >> >> >> You received this message because you are subscribed to the Google >> Groups >> >> "aroma.affymetrix" group with website http://www.aroma-project.org/. >> >> To post to this group, send email to aroma-affymetrix@googlegroups.com >> >> To unsubscribe and other options, go to >> >> http://www.aroma-project.org/forum/ >> > >> > -- >> > When reporting problems on aroma.affymetrix, make sure 1) to run the >> latest >> > version of the package, 2) to report the output of sessionInfo() and >> > traceback(), and 3) to post a complete code example. >> > >> > >> > You received this message because you are subscribed to the Google >> Groups >> > "aroma.affymetrix" group with website http://www.aroma-project.org/. >> > To post to this group, send email to aroma-affymetrix@googlegroups.com >> > To unsubscribe and other options, go to >> http://www.aroma-project.org/forum/ >> > >> >> -- >> When reporting problems on aroma.affymetrix, make sure 1) to run the >> latest version of the package, 2) to report the output of sessionInfo() and >> traceback(), and 3) to post a complete code example. >> >> >> You received this message because you are subscribed to the Google Groups >> "aroma.affymetrix" group with website http://www.aroma-project.org/. >> To post to this group, send email to aroma-affymetrix@googlegroups.com >> To unsubscribe and other options, go to >> http://www.aroma-project.org/forum/ >> > > -- When reporting problems on aroma.affymetrix, make sure 1) to run the latest version of the package, 2) to report the output of sessionInfo() and traceback(), and 3) to post a complete code example. 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