I've done some more tests on the lowess problem.

Please refer from now on to our development server:

http://base.vectorbase.org:8080/base2dev/
Experiment: "Male vs. female (Koutsos et al., 2007)" 

I ran the base1 lowess plugin and got a very different result compared to the
base2 plugin.  The base1 plugin results look correct to me.  The base2
correction factor plots do not look at all right to me.

Can anyone hazard a guess as to if this is a bug or are the default parameters
not suitable for my data?

Due to time pressures I think I'm going to have to switch all our analysis
over to use the base1 plugin.

many thanks,
Bob.

Bob MacCallum writes:
 > 
 > Has anyone had problems with the Lowess analysis plugin?
 > 
 > To me it looks like it is overcorrecting.  Where a M/A plot
 > is largely below the zero line before normalisation, it ends
 > up largely above it afterwards.
 > 
 > I'm using default values (including 0 for block group size).
 > Default values were always fine in BASE1.
 > 
 > You can look for yourself with the guest login at
 > http://fungen.vectorbase.org/base2
 > 
 > Experiment "Male vs. female (Koutsos et al., 2007)" is a good
 > small example.
 > 
 > I don't see a recent ticket on this (I have not upgraded in
 > a few months).
 > 
 > cheers,
 > Bob.
 > 
 > 
 > -- 
 > Bob MacCallum | VectorBase Developer | Kafatos/Christophides Groups |
 > Division of Cell and Molecular Biology | Imperial College London |
 > Phone +442075941945 | Email [EMAIL PROTECTED]
 > 
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-- 
Bob MacCallum | VectorBase Developer | Kafatos/Christophides Groups |
Division of Cell and Molecular Biology | Imperial College London |
Phone +442075941945 | Email [EMAIL PROTECTED]

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