Dear Anant Jain,

Yeah, the problem is that the substitution matrices, e.g., BLOSUM 50, contain the *-symbol. So you'll definitely need the PROTEIN_TERM alphabet when doing protein alignments. Just try and let me know if it works.

Cheers
Andreas Dräger


Dipl.-Bioinform. Andreas Dräger
Eberhard Karls University Tübingen
Center for Bioinformatics (ZBIT)
Sand 1
72076 Tübingen
Germany

Phone: +49-7071-29-70436
Fax:   +49-7071-29-5091

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