So myself & Chris have discussed this off list & we believe it's because of a 
NULL compound element in the Sequence given to the SequenceMixin method.

Does anyone on list know how the AlignedSequence code encodes gaps & the alike? 

Andy

On 6 Dec 2010, at 13:50, Andy Yates wrote:

> Hi Chris,
> 
> Well that's going into my toStringBuilder() method & that particular line is 
> concerned with asking a compound for its String representation. How often do 
> we get nulls in our Sequences and how to deal with them. After all the 
> Sequence AGTCNULLAGTC is probably more harmful then helpful
> 
> Andy
> 
> On 6 Dec 2010, at 12:45, Chris Friedline wrote:
> 
>> Hello,
>> 
>> Found another potential error case, this time in beta2 (fresh pull
>> from git last evening).  For more info, please see
>> http://pastie.org/1351388 for test case and stack trace.  The JUnit
>> test passes simply because the pair object is not null, but fails when
>> trying to extract any information from the pair itself (toString(),
>> getIdenticals(), etc). The substitution matrix file is from
>> ftp://ftp.ncbi.nih.gov/blast/matrices.  I'm doing large numbers of
>> pairwise alignments, which do not all fail, but most do with this same
>> error.
>> 
>> Thanks,
>> Chris
>> 
>> --
>> PhD Candidate, Integrative Life Sciences
>> Virginia Commonwealth University
>> Richmond, VA
>> 
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> 
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-- 
Andrew Yates                   Ensembl Genomes Engineer
EMBL-EBI                       Tel: +44-(0)1223-492538
Wellcome Trust Genome Campus   Fax: +44-(0)1223-494468
Cambridge CB10 1SD, UK         http://www.ensemblgenomes.org/





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