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Dear members,
I am trying to use cryo electron microscope images ( of about 9 A
resolution) to build a search model for molecular replacement trials
(against 2.7 A x-ray diffraction data). As, I dont have any promising
structural homolog for this protein I need to start the model building from
the scratch.
Has any one tried this approach before? Is there any automated software to
build or align secondary structural elements inside an E-M. Image? Please
share your experience and suggestions in this regard.
Thanks in advance
Satheesh
Dr. P.Satheesh Kumar
Postdoctoral Research Associate
Department of Biochemistry and Biophysics
Texas A&M University, 2128 TAMU
College Station, TX - 77843-2128
USA.
Ph. 1-979-862-7639
