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Have you looked at the whole Wilson plot in general, if it has the
right peaks and valleys etc etc ?
You can do that with ccp4/Wilson and its also done by default when you
run ARP/wARP, the latter
having the advantage of seeing the expected shape as established in the
'BEST' work of Popov et al.
133 is not that shocking for a synchrotron dataset (but Tim is right,
be sure what resolution is), I can imagine it can be.
Do you have anisotropy with this huge B? If so, this is one of the
things that Phaser is good at dealing with ...
Good luck.
Tassos
On Nov 17, 2005, at 9:33, Tim Gruene wrote:
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Hi,
maybe you have a low resolution data set (you didn't say. Also some
extract from the data statistics would help). The Wilson plot does not
make sense with resolution worse than say 3.5-4 A.
Tim
- --
Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen
GPG Key ID = A46BEE1A
On Thu, 17 Nov 2005, OnLineHelpForm wrote:
Dear all,
I¡¦m facing a problem with a new data set which collected
from synchrotron. All the statistics of data collection look normal,
only the Wilson plot shows the suggested B factor around 133 !! I¡¦ve
tried to split the data into several parts and scaled each of them
individually, but still didn¡¦t get any improvement. I don¡¦t know is
this the reason that makes me couldn't find the correct solution from
the molecular replacement phasing. Deeply appreciate if anyone has
any suggestion for me.
Best regards,
Yi-Wei Chang
Inst. of Mol. Biol.,
Academia Sinica
Taiwan
[EMAIL PROTECTED]
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