I usually investigate whether there are likely oligamers - dimers,
trimers etc. It is easier to search with a larger unit, if the first 10
seem to be forming such molecules.
Eleanor
Francisco J. Enguita wrote:
*** For details on how to be removed from this list visit the ***
*** CCP4 home page http://www.ccp4.ac.uk ***
Dear All :
I got a pretty difficult case (I guess) of Molecular Replacement. My
protein crystallize in a P21 spacegroup with cell dimensions of around
100 x 200 x 220 A. By Matthews analysis the ASU could accomodate from 16
to 18 molecules of the protein (Molecular weight around 38 kDa). The
resolution of the data is around 2.7 A.
I have run Phaser and it found 10 molecules within the ASU, but the
program failed to find more. I have made and NCS averaging with these
molecules to build up a more precise model, and tried again Phaser with
the same results. I have also tried Molrep with the same results.
Does anybody experienced a problem like this ?. Any suggestion of
strategies to find the rest of the molecules that will complete the
ASU ?.
Any help will be sincerely appreciated.
Cheers
Francisco
begin:vcard
fn:Eleanor Dodson
n:Dodson;Eleanor
email;internet:[EMAIL PROTECTED]
tel;work:+44 (0) 1904 328259
tel;fax:+44 (0) 1904 328266
tel;home:+44 (0) 1904 424449
version:2.1
end:vcard
