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if, in fact, you do have more molecules in your asymmetric unit, then
I would suggest using EPMR to finish your molecular replacement.
This has been very successful for me when I have used a partial
molecular replacement solution locked as a static structure.
Best of luck,
Clint
*******************************************
Paul Clinton Spiegel, Jr., Ph.D.
Jane Coffin Childs Postdoctoral Fellow
Center for Molecular Biology of RNA
University of California, Santa Cruz
Sinsheimer Labs 406
1156 High Street
Santa Cruz, CA 95064
831-459-2700 lab
831-325-3611 cell
http://rna.ucsc.edu/~clint/
On Dec 6, 2005, at 7:16 AM, Francisco J. Enguita wrote:
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Dear All :
I got a pretty difficult case (I guess) of Molecular Replacement. My
protein crystallize in a P21 spacegroup with cell dimensions of around
100 x 200 x 220 A. By Matthews analysis the ASU could accomodate
from 16
to 18 molecules of the protein (Molecular weight around 38 kDa). The
resolution of the data is around 2.7 A.
I have run Phaser and it found 10 molecules within the ASU, but the
program failed to find more. I have made and NCS averaging with these
molecules to build up a more precise model, and tried again Phaser
with
the same results. I have also tried Molrep with the same results.
Does anybody experienced a problem like this ?. Any suggestion of
strategies to find the rest of the molecules that will complete the
ASU ?.
Any help will be sincerely appreciated.
Cheers
Francisco
--
***********************************
Francisco J. Enguita, Ph.D.
Host-pathogen interactions group
Protein Crystallography Laboratory
Av. da Republica
Estação Agronomica Nacional
2780-157 Oeiras
PORTUGAL
Phone : +351-21-4469669
Fax : +351-21-4433644
E-mail : [EMAIL PROTECTED]
Web : http://xtal.itqb.unl.pt
************************************
