HI, Francisco,
I suggest you to check the crystal packing in one unit cell. I like to use TURBO program. you load all your molecules into the program and check if there is any space left for more molecules. The better thing to analyze the crystal packing is to give you a good idea whether you protein will form stable dimer or trimer. You can feed this kind of dimer or trimer into the PHASER to find more solutions.
good luck.
yuequan
At 09:16 AM 12/6/2005, you wrote:
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Dear All :
I got a pretty difficult case (I guess) of Molecular Replacement. My
protein crystallize in a P21 spacegroup with cell dimensions of around
100 x 200 x 220 A. By Matthews analysis the ASU could accomodate from 16
to 18 molecules of the protein (Molecular weight around 38 kDa). The
resolution of the data is around 2.7 A.
I have run Phaser and it found 10 molecules within the ASU, but the
program failed to find more. I have made and NCS averaging with these
molecules to build up a more precise model, and tried again Phaser with
the same results. I have also tried Molrep with the same results.
Does anybody experienced a problem like this ?. Any suggestion of
strategies to find the rest of the molecules that will complete the
ASU ?.
Any help will be sincerely appreciated.
Cheers
Francisco
--
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Francisco J. Enguita, Ph.D.
Host-pathogen interactions group
Protein Crystallography Laboratory
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PORTUGAL
Phone : +351-21-4469669
Fax : +351-21-4433644
E-mail : [EMAIL PROTECTED]
Web : http://xtal.itqb.unl.pt
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