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The recent structure of Dicer was determined from originally twinned crystals
using a unique method. When the crystals were transferred to a lower
concentration of precipitant (PEG) and gently manipulated, they separated into
untwinned shards, which were then transferred back into high PEG cryo and
frozen. I wonder if a destabilizing strategy like that might be generally
applicable towards separating twin domains in other twinned crystals?
A more detailed description (in the supplemental methods) is here
http://www.sciencemag.org/cgi/data/311/5758/195/DC1/1
Jacob
Stefano Benini wrote:
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Dear crystallisators,
I would like to know what people usually tries (possibly succesfully!)
to get not twinned crystals when a protein tends to crystallise as a
perfect twin
I am trying/plannning to try: finding new conditions, additives,
detergents, tag removed, new construct, etc.,
Thank you very much
Ciao
Stefano
**********************************
Stefano Benini Ph.D.
http://www.ysbl.york.ac.uk/~benini
York Structural Biology Laboratory
University of York
Heslington York YO10 5YW United Kingdom
Tel.:+44 1904 328276
Fax: +44 1904 328266
"verba volant scripta manent"
**********************************
--
Jacob Corn
The Berger Lab
UC Berkeley - Molecular and Cell Biology
[EMAIL PROTECTED]
phone: 510-643-8893
fax: 510-643-9290
