*** For details on how to be removed from this list visit the *** *** CCP4 home page http://www.ccp4.ac.uk ***
Hi Stefano, For what it's worth: My homodimeric enzyme formed perfect merohedral twinned crystals. I managed to grow non-twinned cyrstals (using similar conditions) and solve the structure by co-crystallizing with a small domain that bound tightly to the enzyme. Fortunately, the small domain associated across the NCS dyad symmetry axis of the enzyme (in one orientation enforced by crystal packing), which I think prevented twinning from occuring. Good luck, Ren� Frank ======================================================== Department of Biochemistry University of Cambridge United Kingdom CB2 1GA Cell No: +44 7870 208280 Lab No: +44 1223 766019 Fax No: +44 1223 776602 ======================================================== On Thu, 2 Feb 2006, Stefano Benini wrote: > *** For details on how to be removed from this list visit the *** > *** CCP4 home page http://www.ccp4.ac.uk *** > > > > Dear crystallisators, > > I would like to know what people usually tries (possibly succesfully!) > to get not twinned crystals when a protein tends to crystallise as a > perfect twin > > I am trying/plannning to try: finding new conditions, additives, > detergents, tag removed, new construct, etc., > > Thank you very much > > Ciao > Stefano > > ********************************** > Stefano Benini Ph.D. > http://www.ysbl.york.ac.uk/~benini > York Structural Biology Laboratory > University of York > Heslington > York YO10 5YW United Kingdom > Tel.:+44 1904 328276 > Fax: +44 1904 328266 > "verba volant scripta manent" > ********************************** > > >
