Dear All,
We have a protein that can be esaily crystallized, but diffracts very weak.
We tried many mutations/diffrent constructs, the problem is still
there.
Crystals are very beautiful, big, and appear in many conditions with the
same
shape (same space group, too). None of them diffract better than 4 A.
I would appreciate if some one can provide some "fresh" ideas. We have
tried
many things, before crystallization, after crystallization and during data
collection, etc.
Years passed, no impovement. Very frustrating.
Any ideas will be welcome.
Thanks
Rongjin Guan
