*** For details on how to be removed from this list visit the ***
*** CCP4 home page http://www.ccp4.ac.uk ***
I have found that the conditions from 96-well screening kits, such as
Sigma 1 and 2, Nextal, MPD suite, etc., are difficult to replicate in
the 24-well trays, even though a complete description of the chemicals
and concentrations is provided and followed. Many times I have seen
crystalline material or precipitate in a 96-well screening tray, and
obtained nothing when these conditions were replicated into a 24-well
crystallization tray - regardless of the method used to cover / seal the
wells. For sealing I use standard High-Performance Crystal Clear Duck
Tape and it has generally worked well.
.
Mark
swisecar wrote:
*** For details on how to be removed from this list visit the ***
*** CCP4 home page http://www.ccp4.ac.uk ***
Our department recently began using 96 well plates to set up sitting drop
crystallization experiments with a robot. We have had little luck with this
system. When conditions in a 96 well plate were copied by hand in a 24 well
plate we saw differing results (such as clear drops in the 96 well plates and
precipitate or small crystals in the 24 well plates). A sealing film
applicator was used to apply the sealing film. However, we think the problem
may be that each well is not being sealed separately which is impeding proper
vapor diffusion. Has anyone else encountered this problem?
--
_____________________________________
Dr Mark Agacan,
Scientific Officer,
Division of Biological Chemistry
and Molecular Microbiology,
Wellcome Trust Biocenter,
School of Life Sciences, Dow St.,
University of Dundee,
Dundee,
DD1 5EH
Tel: +44 1382 488751
Fax: +44 1382 345764
_____________________________________
