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We have just started using a similar type of setup. The sealing films I have found to not really seal all that well, either from Corning or Griener. I have gotten reasonable results using the Manco Tape packing tape that is typically used for other sitting drop trays. As for going from 96 to 24 well formats, you are increasing the size of your drop and well so the dynamics of the system are going to change. >From what I understand you really have to take your 96 well condition step up the size slowly and also do a fairly fine screen around it. A little work but at least you have a starting point and have been able to screen a wide variety of conditions. On Wed, 2006-08-02 at 10:05 -0400, swisecar wrote: > *** For details on how to be removed from this list visit the *** > *** CCP4 home page http://www.ccp4.ac.uk *** > > > Our department recently began using 96 well plates to set up sitting drop > crystallization experiments with a robot. We have had little luck with this > system. When conditions in a 96 well plate were copied by hand in a 24 well > plate we saw differing results (such as clear drops in the 96 well plates and > precipitate or small crystals in the 24 well plates). A sealing film > applicator was used to apply the sealing film. However, we think the problem > may be that each well is not being sealed separately which is impeding proper > vapor diffusion. Has anyone else encountered this problem? > > -- Leonard M. Thomas Ph.D. Howard Hughes Medical Institute X-ray Laboratory Manager California Institute of Technology [EMAIL PROTECTED] Division of Biology, 114-96 626-395-2453 Pasadena, CA 91125 http://www.br.caltech.edu/cmclab
