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Yes, I've seen that. To me it's sort of a given that 96-well plate will not behave exactly as a 24-well 1ml plate would. Furthermore, glass-sealed Linbros do not behave exactly the same as the nice plastic-sealed Nextal trays. I personally find the 96-well screening very useful, despite the above statement, because I've been usuall able to get crystals *around* the 96-well conditions (but not necessarily at the same exact point). Cheers, Artem =P= > *** For details on how to be removed from this list visit the *** > *** CCP4 home page http://www.ccp4.ac.uk *** > > > Our department recently began using 96 well plates to set up sitting drop > crystallization experiments with a robot. We have had little luck with > this > system. When conditions in a 96 well plate were copied by hand in a 24 > well > plate we saw differing results (such as clear drops in the 96 well plates > and > precipitate or small crystals in the 24 well plates). A sealing film > applicator was used to apply the sealing film. However, we think the > problem > may be that each well is not being sealed separately which is impeding > proper > vapor diffusion. Has anyone else encountered this problem? > > >
