*** For details on how to be removed from this list visit the ***
*** CCP4 home page http://www.ccp4.ac.uk ***
Stefano,
Rather than eliminate NaCl, you can address the problem by adding NaCl
to your reservoir solution.
kmj
Stefano Benini wrote:
*** For details on how to be removed from this list visit the ***
*** CCP4 home page http://www.ccp4.ac.uk ***
Dear All,
I am trying to crystallise a protein that requires 1M NaCl in order to
be happy in solution of about 15 mg/ml (actually with 1M NaCl I can go
much higher than this).
This is influencing my crystallisation trials just because NaCl is
highly hygroscopic
does any of you know which salt (possibly not higher than of 100-200mM)
or wathever I could try to eliminate NaCl and the above mentioned problem?
thank you very much
best regards
Stefano
