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Another happy candidate is the nucleosome protein-DNA complex.
Salting-in is THE procedure to crystallize nucleosomes. We used
salting-in by vapor diffusion (both hanging drops and sitting drops).
Just to give an idea, in our case, if the concentration of the salt in
the stock solution was X, the final concentrations in the drop and
reservoir were X/2 and X/4, respectively.
Good luck.
Raji
[EMAIL PROTECTED] wrote:
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Just a quick note: proteins happily crystallize upon salting-in - for
example Concanavalin A dissolves to 100+ mg/ml in concentrated NaCl, then
crystallizes upon dilution of salt (we did it via dialysis but there are
other ways too).
So you may have your crystallization set-up already!
Good luck,
Artem
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Dear All,
I am trying to crystallise a protein that requires 1M NaCl in order to
be happy in solution of about 15 mg/ml (actually with 1M NaCl I can go
much higher than this).
This is influencing my crystallisation trials just because NaCl is
highly hygroscopic
does any of you know which salt (possibly not higher than of 100-200mM)
or wathever I could try to eliminate NaCl and the above mentioned problem?
thank you very much
best regards
Stefano
--
**********************************
Stefano Benini Ph.D.
http://www.ysbl.york.ac.uk/~benini
York Structural Biology Laboratory
University of York
Heslington
York YO10 5YW United Kingdom
Tel.:+44 1904 328276
Fax: +44 1904 328266
"verba volant scripta manent"
**********************************
--
Raji Edayathumangalam
Postdoctoral Fellow
The Rockefeller University
Box 224. 1230 York Avenue
New York, NY 10021
