Perhaps and idea:
We have a project in which we have a similar problem: need the salt to keep the protein happy. My approach has been to use batch crystallization (no luck yet) to avoid any issues with the salt. Since you make a droplet of known concentrations, you can (if you wish) keep the salt in there at the concentration you know you need to keep the protein happy and this will not change over time.
My 2 cents worth!
Mark
We have a project in which we have a similar problem: need the salt to keep the protein happy. My approach has been to use batch crystallization (no luck yet) to avoid any issues with the salt. Since you make a droplet of known concentrations, you can (if you wish) keep the salt in there at the concentration you know you need to keep the protein happy and this will not change over time.
My 2 cents worth!
Mark
-----Original Message-----
From: [EMAIL PROTECTED]
To: [email protected]
Sent: Thu, 9 Nov 2006 5:35 AM
Subject: [ccp4bb]: using salts to improve protein solubility/stability
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Dear All,
I am trying to crystallise a protein that requires 1M NaCl in order to be happy in solution of about 15 mg/ml (actually with 1M NaCl I can go much higher than this).
This is influencing my crystallisation trials just because NaCl is highly hygroscopic
does any of you know which salt (possibly not higher than of 100-200mM) or wathever I could try to eliminate NaCl and the above mentioned problem?
thank you very much
best regards
Stefano
--
**********************************
Stefano Benini Ph.D.
http://www.ysbl.york.ac.uk/~benini
York Structural Biology Laboratory
University of York
Heslington York YO10 5YW United Kingdom
Tel.:+44 1904 328276
Fax: +44 1904 328266
"verba volant scripta manent"
**********************************
*** CCP4 home page http://www.ccp4.ac.uk ***
Dear All,
I am trying to crystallise a protein that requires 1M NaCl in order to be happy in solution of about 15 mg/ml (actually with 1M NaCl I can go much higher than this).
This is influencing my crystallisation trials just because NaCl is highly hygroscopic
does any of you know which salt (possibly not higher than of 100-200mM) or wathever I could try to eliminate NaCl and the above mentioned problem?
thank you very much
best regards
Stefano
--
**********************************
Stefano Benini Ph.D.
http://www.ysbl.york.ac.uk/~benini
York Structural Biology Laboratory
University of York
Heslington York YO10 5YW United Kingdom
Tel.:+44 1904 328276
Fax: +44 1904 328266
"verba volant scripta manent"
**********************************
