*** For details on how to be removed from this list visit the *** *** CCP4 home page http://www.ccp4.ac.uk ***
Just a quick note: proteins happily crystallize upon salting-in - for example Concanavalin A dissolves to 100+ mg/ml in concentrated NaCl, then crystallizes upon dilution of salt (we did it via dialysis but there are other ways too). So you may have your crystallization set-up already! Good luck, Artem > *** For details on how to be removed from this list visit the *** > *** CCP4 home page http://www.ccp4.ac.uk *** > > > Dear All, > > I am trying to crystallise a protein that requires 1M NaCl in order to > be happy in solution of about 15 mg/ml (actually with 1M NaCl I can go > much higher than this). > > This is influencing my crystallisation trials just because NaCl is > highly hygroscopic > > does any of you know which salt (possibly not higher than of 100-200mM) > or wathever I could try to eliminate NaCl and the above mentioned problem? > > thank you very much > best regards > > Stefano > > -- > > ********************************** > Stefano Benini Ph.D. > http://www.ysbl.york.ac.uk/~benini > York Structural Biology Laboratory > University of York > Heslington > York YO10 5YW United Kingdom > Tel.:+44 1904 328276 > Fax: +44 1904 328266 > "verba volant scripta manent" > ********************************** > > >
