Stirred cell from Milipore is very useful for concentrating big volumes of protein solution. There are cells from 3 to 400 mL. Ultrafiltration is performed through the membrane with high pressure by connecting to inert gas (nitrogen). It is very fast and comfortable technique. Here are more information: http://www.millipore.com/catalogue/module/C3259
Sincerely, Radovan Spurny, PhD Laboratory of Structural Neurobiology Division of Pharmacology Campus Gasthuisberg, ON1 Herestraat 49, PB 601 B-3000 Leuven Belgium e-mail: radovan.spu...@med.kuleuven.be ________________________________________ From: CCP4 bulletin board [ccp...@jiscmail.ac.uk] On Behalf Of Yingyun Liu [yingyun...@jhu.edu] Sent: Thursday, September 03, 2009 16:48 To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Devices Suitable to Concentrate Protein Solutions in Liter Scale An anion exchange column with a large enough bed volume might be used to concentrate the protein. You probably need to change the pH of the solution so that the protein sticks to the column. On Thu, 2009-09-03 at 17:05 +0800, Xuan Yang wrote: > Dear Dr Ku, > > It was a wonderful idea! However, the refolding buffer contained 500mM > L-Arg and 1mM EDTA. > > And I want to try other affinity columns, just don't know what resin > would be appropriate. > > Sincerely, > > Xuan Yang > > > 2009/9/3 Shao-Yang Ku <s...@embl-hamburg.de> > Can you put a 6His-tag on your protein and add some Ni-NTA > beads to your solution to capture (and concentrate) the > properly folded molecules? > > > > Quoting "Xuan Yang" <pattisy...@gmail.com>: > > Dear All, > > I am working on protein refolding via dialysis in > large volumn > (typically 2~4 litters). It was problematic when I > wanted to concentrate the > solution to at least less than 500ml. If you know any > device appropriate for > such task, please help me out:) > > Thanks in advance! > > Sincerely, > > Xuan Yang > > > >
