Hi,
Could you not carry out Ammonium Sulfate precipitation to concentrate the
protein? I never did it myself so I have no idea if it's practical in this
case. Maybe it's still worth mentioning, though.
Tim
--
Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen
GPG Key ID = A46BEE1A
On Fri, 4 Sep 2009, Radovan Spurny wrote:
Stirred cell from Milipore is very useful for concentrating big volumes of
protein solution. There are cells from 3 to 400 mL. Ultrafiltration is
performed through the membrane with high pressure by connecting to inert gas
(nitrogen). It is very fast and comfortable technique. Here are more
information: http://www.millipore.com/catalogue/module/C3259
Sincerely,
Radovan Spurny, PhD
Laboratory of Structural Neurobiology
Division of Pharmacology
Campus Gasthuisberg, ON1
Herestraat 49, PB 601
B-3000 Leuven
Belgium
e-mail: radovan.spu...@med.kuleuven.be
________________________________________
From: CCP4 bulletin board [ccp...@jiscmail.ac.uk] On Behalf Of Yingyun Liu
[yingyun...@jhu.edu]
Sent: Thursday, September 03, 2009 16:48
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Devices Suitable to Concentrate Protein Solutions in
Liter Scale
An anion exchange column with a large enough bed volume might be used to
concentrate the protein. You probably need to change the pH of the
solution so that the protein sticks to the column.
On Thu, 2009-09-03 at 17:05 +0800, Xuan Yang wrote:
Dear Dr Ku,
It was a wonderful idea! However, the refolding buffer contained 500mM
L-Arg and 1mM EDTA.
And I want to try other affinity columns, just don't know what resin
would be appropriate.
Sincerely,
Xuan Yang
2009/9/3 Shao-Yang Ku <s...@embl-hamburg.de>
Can you put a 6His-tag on your protein and add some Ni-NTA
beads to your solution to capture (and concentrate) the
properly folded molecules?
Quoting "Xuan Yang" <pattisy...@gmail.com>:
Dear All,
I am working on protein refolding via dialysis in
large volumn
(typically 2~4 litters). It was problematic when I
wanted to concentrate the
solution to at least less than 500ml. If you know any
device appropriate for
such task, please help me out:)
Thanks in advance!
Sincerely,
Xuan Yang
