Hi Wataru: I hope all is well. For the ones you already have grown, try very gently prying them off with a wedge-shaped needle.
If you can grow more, try using a very thin smooth layer of vacuum grease, and apply the drop to that. I managed to get RNA crystals to grow that way that otherwise irreversibly adhered to the surface. All the best, Bill On Feb 8, 2011, at 6:06 PM, Wataru Kagawa wrote: > Hi all, > > I have crystals growing by the hanging-drop method, using 24-well VDX plates > and Hampton Research siliconized glass cover slides. Most crystals are > attached to the cover slide, and I am having difficulties detaching the > crystals (using a cryoloop) without breaking them. There are few smaller > crystals floating in the drop, and they diffract X-rays pretty well (clean > spots, ~3.5A resolution using RAXIS). However, I would like to try the bigger > ones, because they may diffract to a higher resolution. > > Any suggestions for detaching crystals from cover slides will be greatly > appreciated. > > Wataru
