Dear Wataru check out the following entry on the CCP4-wiki on 'sticky crystals' which we compiled based on feeback from many crystallographers:
http://strucbio.biologie.uni-konstanz.de/ccp4wiki/index.php/Sticky_crystals Best regards Savvas ---- Savvas Savvides Unit for Structural Biology @ L-ProBE Ghent University K.L. Ledeganckstraat 35, 9000 Ghent, Belgium Tel/SMS/texting +32 (0)472 928 519 Skype: savvas.savvides_skype http://www.LProBE.ugent.be/xray.html On 09 Feb 2011, at 16:11, Colbert, Christopher wrote: > Hi Wataru, > > I second Bill's suggestion. Additionally, you might want to migrate to a > sitting drop setup and use the vacuum grease with that. It worked for me. > > Happy Fishing, > > Chris > > -- > Christopher L. Colbert, Ph.D. > Assistant Professor > Department of Chemistry and Biochemistry > North Dakota State University > P.O. Box 6050 Dept. 2710 > Fargo, ND 58108-6050 > PH: (701) 231-7946 > FAX: (701) 231-8324 > > > > > > On 2/8/11 8:19 PM, "William G. Scott" <[email protected]> wrote: > >> Hi Wataru: >> >> I hope all is well. For the ones you already have grown, try very gently >> prying them off with a wedge-shaped needle. >> >> If you can grow more, try using a very thin smooth layer of vacuum >> grease, and apply the drop to that. I managed to get RNA crystals to >> grow that way that otherwise irreversibly adhered to the surface. >> >> All the best, >> >> Bill >> >> >> On Feb 8, 2011, at 6:06 PM, Wataru Kagawa wrote: >> >>> Hi all, >>> >>> I have crystals growing by the hanging-drop method, using 24-well VDX >>> plates and Hampton Research siliconized glass cover slides. Most >>> crystals are attached to the cover slide, and I am having difficulties >>> detaching the crystals (using a cryoloop) without breaking them. There >>> are few smaller crystals floating in the drop, and they diffract X-rays >>> pretty well (clean spots, ~3.5A resolution using RAXIS). However, I >>> would like to try the bigger ones, because they may diffract to a higher >>> resolution. >>> >>> Any suggestions for detaching crystals from cover slides will be >>> greatly appreciated. >>> >>> Wataru >> >
