Hi Wataru, I second Bill's suggestion. Additionally, you might want to migrate to a sitting drop setup and use the vacuum grease with that. It worked for me.
Happy Fishing, Chris -- Christopher L. Colbert, Ph.D. Assistant Professor Department of Chemistry and Biochemistry North Dakota State University P.O. Box 6050 Dept. 2710 Fargo, ND 58108-6050 PH: (701) 231-7946 FAX: (701) 231-8324 On 2/8/11 8:19 PM, "William G. Scott" <[email protected]> wrote: >Hi Wataru: > >I hope all is well. For the ones you already have grown, try very gently >prying them off with a wedge-shaped needle. > >If you can grow more, try using a very thin smooth layer of vacuum >grease, and apply the drop to that. I managed to get RNA crystals to >grow that way that otherwise irreversibly adhered to the surface. > >All the best, > >Bill > > >On Feb 8, 2011, at 6:06 PM, Wataru Kagawa wrote: > >> Hi all, >> >> I have crystals growing by the hanging-drop method, using 24-well VDX >>plates and Hampton Research siliconized glass cover slides. Most >>crystals are attached to the cover slide, and I am having difficulties >>detaching the crystals (using a cryoloop) without breaking them. There >>are few smaller crystals floating in the drop, and they diffract X-rays >>pretty well (clean spots, ~3.5A resolution using RAXIS). However, I >>would like to try the bigger ones, because they may diffract to a higher >>resolution. >> >> Any suggestions for detaching crystals from cover slides will be >>greatly appreciated. >> >> Wataru >
