Hi Alex, Most DNA-binding proteins has decent affinity to heparin columns. Have you tried purifying your protein over heparin columns?
Cheers, Raji On Sat, Apr 9, 2011 at 8:44 PM, Alexandra Deaconescu <[email protected]>wrote: > Hello ccp4 enthusiasts: > > I am afraid this is a non-ccp4 related question. Can anyone recommend an > immobilized dsDNA chromatographic resin for purification of DNA-binding > proteins? GE seems to have something - I was wondering if people have other > recommendations? In the age of GST and His tags etc., these are not very > much used, but I do not have a tag in this case... > > Thanks a lot, > Alex > -- ----------- Raji Edayathumangalam Research Fellow in Neurology, Harvard Medical School Postdoctoral Fellow, Center for Neurologic Diseases, Brigham and Women's Hospital Visiting Research Scholar, Brandeis University
