Related fact I learned recently, perhaps of interest for fellow record-keepers:
heparin sulfate has the highest negative charge density of any known biological molecule. JPK On Sat, Apr 9, 2011 at 9:38 PM, Raji Edayathumangalam <r...@brandeis.edu> wrote: > Hi Alex, > > Most DNA-binding proteins has decent affinity to heparin columns. Have you > tried purifying your protein over heparin columns? > > Cheers, > Raji > > > > On Sat, Apr 9, 2011 at 8:44 PM, Alexandra Deaconescu <deac...@brandeis.edu> > wrote: >> >> Hello ccp4 enthusiasts: >> >> I am afraid this is a non-ccp4 related question. Can anyone recommend an >> immobilized dsDNA chromatographic resin for purification of DNA-binding >> proteins? GE seems to have something - I was wondering if people have other >> recommendations? In the age of GST and His tags etc., these are not very >> much used, but I do not have a tag in this case... >> >> Thanks a lot, >> Alex > > > > -- > > ----------- > Raji Edayathumangalam > Research Fellow in Neurology, Harvard Medical School > Postdoctoral Fellow, Center for Neurologic Diseases, Brigham and Women's > Hospital > Visiting Research Scholar, Brandeis University > > -- ******************************************* Jacob Pearson Keller Northwestern University Medical Scientist Training Program cel: 773.608.9185 email: j-kell...@northwestern.edu *******************************************
