Hi Alex, as you have a DNA binding protein does it have a high pI? If so, good old ion exchange with an S-sepharose column should do it.
Cheers James Dr James Garnett Division of Molecular Biosciences, Imperial College London, Level 5, Biochemistry Building, South Kensington, LONDON SW7 2AZ, UK. Tel: +44 (0) 207 594 5464 Fax: +44 (0) 207 594 3057 ----- Reply message ----- From: "Raji Edayathumangalam" <r...@brandeis.edu> To: "CCP4BB@JISCMAIL.AC.UK" <CCP4BB@JISCMAIL.AC.UK> Subject: [ccp4bb] immobilized DNA resin Date: Sun, Apr 10, 2011 03:38 Hi Alex, Most DNA-binding proteins has decent affinity to heparin columns. Have you tried purifying your protein over heparin columns? Cheers, Raji On Sat, Apr 9, 2011 at 8:44 PM, Alexandra Deaconescu <deac...@brandeis.edu<mailto:deac...@brandeis.edu>> wrote: Hello ccp4 enthusiasts: I am afraid this is a non-ccp4 related question. Can anyone recommend an immobilized dsDNA chromatographic resin for purification of DNA-binding proteins? GE seems to have something - I was wondering if people have other recommendations? In the age of GST and His tags etc., these are not very much used, but I do not have a tag in this case... Thanks a lot, Alex -- ----------- Raji Edayathumangalam Research Fellow in Neurology, Harvard Medical School Postdoctoral Fellow, Center for Neurologic Diseases, Brigham and Women's Hospital Visiting Research Scholar, Brandeis University
