Jun Yong and Jobichen (I've mentioned this before, but ) - both of your projects jump out as very good targets for microseeding with random screens. This method often gives extra hits and better crystals because it is more likely that crystals will grow in the metastable zone. It often reduces the *need *for optimization.
Read Allan D'Arcy's excellent paper, plus Obmolova et al for a spectacular example. Our web site has some recent tips that may help you too. Good luck Patrick D'Arcy, A., Villard, F., Marsh, M. "An automated microseed matrix screening method for protein crystallization", 2007, Acta Crystallographica, D63, 550-554. G. Obmolova, T. J. Malia, A. Teplyakov, R. Sweet and G. L. Gilliland. Promoting crystallization of antibody-antigen complexes via microseed matrix screening Acta Cryst. (2010). D66, 927-933 http://www.douglas.co.uk/mms.htm * * On Tue, Apr 12, 2011 at 1:07 PM, Jun Yong Ha <[email protected]> wrote: > Hi all, > > Recently, I produced crystals with MBClass1-64 which contains PEG4000, > HEPES-Na and NaCl. But, I struggled to reproduce crystals. I tried to set up > tray with different batch of solution. I got the crystals only from 2008 > solution, but not from fresh ones. I asked technical service of Qiagen, but > they did not have any stock. > > pH between fresh and old solution is the same. I could reproduce crystals > with this old solution 100% when setting up. > > Do you have any experience like this? Is PEG4000 degraded or oxidized? > > Please help me. > > Thanks in advance. > -- [email protected] Douglas Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090 US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36
