Dear Israel,
There is a lot to look up in this field. I believe it started with
Schick, B. & Jurnak, F. (1994). Acta Cryst. D50, 563-568.
at http://scripts.iucr.org/cgi-bin/paper?gr0289
There is a nice review online at
http://www-bio3d-igbmc.u-strasbg.fr/~mgsb/biophys/rx/biblio/heras_2005.pdf
and then of course there has been the development of the Free-Mounting
System by Proteros:
http://www.proteros.com/articles.php?sid=18&lang=de
based on a different method of hydration control.
Good luck!
Gerard.
--
On Sun, May 01, 2011 at 06:32:21PM +0100, Israel Sanchez wrote:
> Hi folks,
>
>
> I am currently impressed by the efficiency of dehydration treatments over
> the diffraction capacity of our crystals in one particular condition.
> Without any treatment the crystals seldom diffract to 20-30A but in our last
> synchrotron trip the very same crystals, after been incubated with
> increasing concentration of low molecular weight PEGs diffracted to 6A.
>
> I was wondering if anyone has studied these effects in a systematic way.
> Does anyone on the ccp4bb knows references or has any
> experience/pseudo-religious believes that do not care to share with the
> community about this particular topic?
>
>
> Thank you very much in advance!!!!
>
>
> --
> Israel Sanchez Fernandez PhD
> Ramakrishnan-lab
> MRC Laboratory of Molecular Biology,
> Hills Road, Cambridge, CB2 0QH, UK
--
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