Dear Israel,
there is also a dedicated humidity device developed at EMBL/ESRF
Grenoble that allows for systematic dehydration experiments to be
carried out at room temperature and soon at 4°C. Here is the primary
reference:
Sanchez-Weatherby, J., Bowler, M.W., Huet, J., Gobbo, A., Felisaz, F.,
Lavault, B., Moya, R., Kadlec, J., Ravelli, R.B. and Cipriani, F. (2009)
Improving diffraction by humidity control: a novel device compatible
with X-ray beamlines. Acta Crystallogr D Biol Crystallogr., 65, 1237-1246.
Cheers,
Martin
Le 01/05/11 19:32, Israel Sanchez a écrit :
Hi folks,
I am currently impressed by the efficiency of dehydration treatments
over the diffraction capacity of our crystals in one particular
condition. Without any treatment the crystals seldom diffract to
20-30A but in our last synchrotron trip the very same crystals, after
been incubated with increasing concentration of low molecular weight
PEGs diffracted to 6A.
I was wondering if anyone has studied these effects in a systematic
way. Does anyone on the ccp4bb knows references or has any
experience/pseudo-religious believes that do not care to share with
the community about this particular topic?
Thank you very much in advance!!!!
--
Israel Sanchez Fernandez PhD
Ramakrishnan-lab
MRC Laboratory of Molecular Biology,
Hills Road, Cambridge, CB2 0QH, UK
