Hi Israel, Just to add to that, and as I know you use the Diamond synchrotron, the device mentioned is available (on advance request) on the beamline I02.
Cheers -- David On 1 May 2011 19:50, Martin Weik <[email protected]> wrote: > Dear Israel, > > there is also a dedicated humidity device developed at EMBL/ESRF Grenoble > that allows for systematic dehydration experiments to be carried out at room > temperature and soon at 4°C. Here is the primary reference: > > Sanchez-Weatherby, J., Bowler, M.W., Huet, J., Gobbo, A., Felisaz, F., > Lavault, B., Moya, R., Kadlec, J., Ravelli, R.B. and Cipriani, F. (2009) > Improving diffraction by humidity control: a novel device compatible with > X-ray beamlines. Acta Crystallogr D Biol Crystallogr., 65, 1237-1246. > > Cheers, > > Martin > > > Le 01/05/11 19:32, Israel Sanchez a écrit : > > Hi folks, >> >> >> I am currently impressed by the efficiency of dehydration treatments over >> the diffraction capacity of our crystals in one particular condition. >> Without any treatment the crystals seldom diffract to 20-30A but in our last >> synchrotron trip the very same crystals, after been incubated with >> increasing concentration of low molecular weight PEGs diffracted to 6A. >> >> I was wondering if anyone has studied these effects in a systematic way. >> Does anyone on the ccp4bb knows references or has any >> experience/pseudo-religious believes that do not care to share with the >> community about this particular topic? >> >> >> Thank you very much in advance!!!! >> >> >> -- >> Israel Sanchez Fernandez PhD >> Ramakrishnan-lab >> MRC Laboratory of Molecular Biology, >> Hills Road, Cambridge, CB2 0QH, UK >> >> >>
