-----BEGIN PGP SIGNED MESSAGE----- Hash: SHA1 Dear Len,
just to be on the safe side, my list of 'usual suspects' includes - - glycerol/PEG400 - - LiCl et al at high concentration - - Butanediol - - sugars (glucose/ fructose) - - oil - - NaMalonate - - MPD ... you mention cracking upon transferring the crystal. - - do you use a pipet for transfer? - - addition of cryo TO the drop? - - did you try slow (several minutes - 1hr) / quick addition of cryo protectant - - seeding into slightly different conditions/additive screens - - seeding into cryo conditions ... How about collecting data at room temperature? Hope this list contains some new ideas. Best wishes, Tim On 10/26/2011 06:46 PM, Leonard Thomas wrote: > Hi All, > > I have run into a very sensitive crystals system when it comes to cryo > protecting them. I have run through the usual suspects and trays are > going to be setup with a cryo protectant as part of crystallization > cocktail. The one problem that seems to be occurring is that the > crystals crack as soon as they are transfered out of the original drop. > I am running out of ideas and really would love some new ones. > > Thanks in advance. > > Len > > Leonard Thomas Ph.D. > Macromolecular Crystallography Laboratory Manager > University of Oklahoma > Department of Chemistry and Biochemistry > Stephenson Life Sciences Research Center > 101 Stephenson Parkway > Norman, OK 73019-5251 > > [email protected] > http://barlywine.chem.ou.edu > Office: (405)325-1126 > Lab: (405)325-7571 > - -- - -- Dr Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A -----BEGIN PGP SIGNATURE----- Version: GnuPG v1.4.10 (GNU/Linux) Comment: Using GnuPG with Mozilla - http://enigmail.mozdev.org/ iD8DBQFOqD5+UxlJ7aRr7hoRAlFlAJ9b4ieJzoX5J6RRce85Si05d/pkFACdEQGR GAh002nC1bod6VCBolOv3pQ= =IO/R -----END PGP SIGNATURE-----
